Guidelines for the antidiabetic assay for bioactive substances in cell model

Diabetes is a group of metabolic diseases characterized by hyperglycemia. Insulin resistance (IR) and insufficient insulin secretion are important pathophysiological basis significant characteristics diabetes. At present, animal still the main model for investigation Antidiabetic activity bioactive substances, however, in vivo models always has problems long experimental cycle, complicated experiment, high cost. Compared with models, cell culture could avoid some impossible or difficult to eliminate under natural conditions, observe results more simply, economically, quickly, objectively. In this case, establishment vitro diabetic gradually developed. Recently, widely used high-throughput screening, metabolism analysis, molecular pharmacology investigation, pharmacodynamics evaluation product, function, interaction between receptors cytokines, so on. These guidelines will point out discuss help researchers choose that truly reliable can be successfully applied their intended antidiabetic activity.